Tk6 lymphoblastoid human cells 微核

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WebDec 1, 2015 · TK6 cells, a human lymphoblastoid cell line were obtained from American Type Culture Collection (ATCC# CRL-8015; ATCC, Manassas, VA, USA). Briefly, cells were cultured and maintained in RPMI 1640 medium containing 10% heat inactivated horse serum, in addition to 0.1% pluronics, sodium pyruvate and antibiotics (penicillin at 20 … WebMar 29, 2024 · In a previous study by this group, we constructed a model of malignant transformation of human lymphoblastoid cells (TK6) induced by chronic exposure to HQ with significant subcutaneous tumorigenic capacity in nude mice. miR-92a-3p is a tumor factor whose role in HQ-induced malignant transformation is not yet clear. In the present …

methods for human lymphoblast TK6 cell line Comparison …

WebThe implementation of magnetic resonance imaging (MRI) guided radiotherapy (RT) continues to increase. Very limited in-vitro data on the interaction of ionizing radiation and … WebMar 19, 2007 · Experiments were performed to investigate the impact of didanosine (ddI), lamivudine (3TC), and stavudine (d4T) on cell survival and mutagenicity in two reporter genes, hypoxanthine-guanine phosphoribosyltransferase (HPRT) and thymidine kinase (TK), using a cell cloning assay for assessing the effects of individual nucleoside analogs … tinto road bearsden

In Vitro PIG-A Gene Mutation Assay in Human B …

WebTK6 human lymphoblastoid cells are widely used in the in vitro MN assay as a part of regulatory genotoxicity testing for pharmaceuticals, but have seen relatively limited use in testing of other test materials, including tobacco-related products. WebFeb 1, 1995 · The U.S. Department of Energy's Office of Scientific and Technical Information The relative radiosensitivity of TK6 and W1-L2-NS lymphoblastoid cells derived from a common source is primarily determined by their p53 mutational status. (Journal Article) OSTI.GOV skip to main content Sign In Create Account Show searchShow menu WebCell Culture Cells of the human lymphoblastoid cell line TK6 were provided by W. G. Thilly of the Massachusetts Institute of Technology. Both the TK6 and WTK1 cell lines are TK-'- descendants of the WI-L2 cell line. The isolation of the TK6 and WTK1 cell lines has been reviewed by Amund-son et al. (34). The cells were grown in suspension at ... tint or gloss treatment

Human Lymphoblastoid Tk6 Cell Line ATCC Bioz

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WebNov 1, 2004 · TK6 is a human B lymphoblastoid cell line that was isolated from a spontaneous immortalized lymphoblast culture WIL2 in 1968 (Levy et al., 1968). TK6 was … WebJialong Chen is an academic researcher from Southern Medical University. The author has contributed to research in topic(s): DNA methylation & Trichostatin A. The author has an hindex of 4, co-authored 7 publication(s) receiving 38 citation(s). password powerpoint removeWebWIL2-NS and TK6 are two distinct human lymphoblast cell lines derived from a single male donor. WIL2-NS cells are significantly more resistant to the cytotoxic effects of X-irradiation but considerably more sensitive to induced mutation. tint or red is

"WebFeb 20, 2024 · Here, TK6 cells exhibited 17.2 ± 4.5 micronucleated cells per 1,000 binucleated cells. HSC showed a lower frequency of 5.5 ± 2.8 micronucleated cells per 1,000 binucleated cells... " - Tk6 lymphoblastoid human cells 微核

Tk6 lymphoblastoid human cells 微核

The influence of a magnetic field on photon beam radiotherapy in …

WebJun 1, 2024 · The human lymphoblastoid TK6 cell line was established in 1978 from the parent cell line WI-L2 that was derived from a 5-year-old male with hereditary … WebJul 4, 2012 · The Organization for Economic Co-operation and Development (OECD) has recently adopted Test Guideline 487 (TG487) for conducting the in vitro micronucleus …

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WebThe TK6 Micronucleus (MN) Assay TK6 cells are derived from a human B lymphoblastoid cell line. Therefore, they are p53 proficient and karyotypically stable. The TK6 … WebTK6. CRL-8015 ™. TK6 is a lymphoblast cell line that was isolated from the spleen of a 5-year-old, male with hereditary spherocytosis. The cells are heterozygous at the thymidine …

WebTK6 cells are human origin and express wild-type p53 protein. These properties could suggest that the genotoxic and cytotoxic response of TK6 cells against chemical substances are more relevant to intact human cells than that of other mammalian cells. It could be advantageous to consider human hazard and risk. WebThe uncovering of genes involved in susceptibility to the sporadic cancer types is a great challenge. It is well established that the way in which an individual deals with DNA damage is related to th

WebFor the ICD analysis, Jurkat and TK6 cells were seeded in a 24-well plate in RPMI. The ICD-relevant surface proteins, CD47, PD-L1, HSP90, and CRT, were measured via flow cytometry. WebAug 1, 1995 · TK6 is a human cell line developed for gene mutation assays and thus may have advantages for human hazard identification and assessment. On the other hand, …

WebApr 1, 2024 · The human lymphoblastoid TK6 cell lines were grown in RPMI-1640 medium (NacalaiTesque) supplemented with 10% heat-inactivated horse serum (Nichirei Biosciences, Inc.), 200 μg/mL sodium pyruvate ...

WebDec 1, 2015 · In this study, TK6 cells were exposed to increasing doses of four chemicals (two genotoxic that require metabolic activation and two non-genotoxic chemicals) in the … tintor screamingWebATCC human lymphoblastoid tk6 cell line Human Lymphoblastoid Tk6 Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more tint or shade crossword clueWebIn the presence of an activating system, ethyl carbamate was found to be mutagenic in Salmonella typhimurium strain TA100 but not in strains TA98 and TA102, indicating base-pair substitutions at G-C base pairs. In contrast, no significant mutagenicity of ethyl carbamate could be detected in human lymphoblastoid TK6 cells. tint or shadeWebThe implementation of magnetic resonance imaging (MRI) guided radiotherapy (RT) continues to increase. Very limited in-vitro data on the interaction of ionizin password printer epson l3150WebTK6 human lymphoblastoid cells are widely used in the in vitro MN assay as a part of regulatory genotoxicity testing for pharmaceuticals, but have seen relatively limited use in … tinto sachunterrichtWebJan 1, 2016 · Abstract. Closely related TK6, WTK1, and NH32 human B-lymphoblastoid cell lines differ in their p53 functional status. These lines are used frequently in genotoxicity … tint ortigasWebJul 4, 2012 · TK6 cells are suitable for the MNvit assay in accordance with OECD guideline TG487. Extended recovery period after treatment improves sensitivity of TK6 cells. Negative controls showed reproducibly low and consistent micronucleus frequencies. MNvit assay in TK6 cells clearly differentiates negative and positive compounds. MNvit assay in … tint or shade crossword