Splet20. jul. 2024 · Preliminary experiments conducted in a previous study showed that the FnCas12a two-plasmid systems pCpf1-RK2K/pSEVA-gcR3361T and pCpf1-RK2K/pSEVA-gcR5301T were able to perform deletion in P. putida (Sun et al., 2024). On this basis, we explored the possibility to construct a FnCas12a-based genome editing system for P. … Splet13. jan. 2024 · In the second genome editing experiment, all 43 F1-T0 plants transformed with pCpf1_gRNA2 were assayed for gRNA activity (Supplementary Fig. 1b, Supplementary Data 1) and selected for reciprocal ...
Single-stranded gaps in plasmids reduce cloning efficiency. A ...
Splet19. nov. 2024 · Through End With Adhesive Tape of Mirai Industry,PCPF1, Check out the variety of configurable, Through End With Adhesive Tape of Mirai Industry,PCPF1,MISUMI has other mechanical components, Press Die, and Plastic Mold products available. MISUMI offers free CAD download, short lead times, competitive pricing, and no minimum order … SpletWere every criminal violation the causal consequence of some willed action, then something very much like (PF1) would describe the prima facie case. うさぎ 漢字
SEVA-Cpf1, a CRISPR-Cas12a vector for genome editing in …
Splet03. apr. 2024 · The PCPF-1@SWAT2012 model is capable of well simulating the water flow rate and transport of herbicides in given watershed, comprising different land use types, … Splet10. dec. 2024 · developed another vector, pCpf1-Sp, which was derived from pCpf1 following replacement of the kanamycin-resistance cassette nptII by the … SpletPF1, PF-1, PF01, PF-01, or variant, may refer to: PSA PF1 platform, the "PF1" platform from PSA. pf1, a bacteriophage code, see List of MeSH codes (B04) Pf1, a Pseudomonas … うさぎ 無料 年賀状